DNA
Message

Part:BBa_K361001:Design

Designed by: Jacky, Fong Chuen, Loo   Group: iGEM10_Hong_Kong-CUHK   (2010-10-24)


Message gene template


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 404
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 7
    Illegal BamHI site found at 427
    Illegal XhoI site found at 433
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This DNA sequence is acting as a template, to guide the make of a message inserted with specific repeat sequence for recognition by Rci recombinase in order to accomplish random rearrangement of message in encryption process.


Source

This artificial DNA sequence is converted from text with the use of ASCII system.

The specific repeat sequence "repeat 1" and "repeat 2" are come from transfer region in R64 plasmid, with genomic assession no.:AB027308.1

References